The stand-up cut short his set at the Comcast Theater in Hartford, Connecticut, after getting fed up with members of the audience shouting at him and quoting his older gags.
Oswalt has now taken to Twitter.com to urge those with tickets to Chappelle's ongoing comeback tour to let him do what he does best.
He writes, "Dear Dave Chappelle's fans: He's one of the best comedians working, & you're not letting him do what he loves. Stop being a**holes. Chappelle's touring now, trying to do his usual, brilliant stuff, and the crowds are screaming, 'I'm Rick James, b**ch!' Idiots...
"If you INTERRUPT a performer, f**k up their flow & timing, even in praise, you're INSULTING THEM. Good Lord..."
Chappelle's Funny Or Die tour, which also features comedy duo Flight of the Conchords, continues at Tinley Park, Illinois, on Saturday.